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标题：Biodegradation of crude oil using efficient biosurfactant producing microorganisms
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作者：Prabha A. Soma ; A. Jayachitra
期刊名称：Research Journal of Recent Sciences
电子版ISSN：2277-2502
出版年度：2018
卷号：7
期号：9
页码：1-6
语种：English
出版社：International Science Community Association
摘要：Pollution of sea by crude oil caused by stranding of tankers is one of the urgent and serious environment issues over the world. Biosurfactant are formed as extracellular compounds or localized on cell surface of microorganisms. Therefore in the present study focus of attention was given with the foresight of using biosurfactant as a promising tool to emulsify the polluted oils prior to biodegradation. When microbes grow in hydrocarbon contaminated site it undergoes may adaptations. It influences the uptake of hydrocarbon as substrate. Crude biosurfactant helps the biodegradation of hydrocarbon using biosurfactnat producing bacteria to gain better access to their hydrophobic substrates since it bring reduction of surface tension of the marine ecosystem around the bacterium. In the present study, crude oil degradation with mineral salt medium (MSM) the Infrared spectrum of Pseudomonas aeruginosa, Pseudomonas stutzeri, Bacillus cereus, Bacillus licheniformis and control which produce biosurfactant was confirmed by FTIR analysis. The result on bacterial cell growth in all the experiments revealed that biosurfactant producing bacterial cells utilized crude oil as the carbon and energy source which was evident from cell growth observed with experimental Set up exhibited by extraction of crude oil revealed 81.4%, 85.6%, 77.2% and 68.3% of crude oil biodegradation by Pseudomonas aeruginosa, Pseudomonas stutzeri, Bacillus cereus, Bacillus licheniformis, standard and control. Synthetic biosurfactant as standard n-hexane revealed 88.6% during biodegradation of crude oil and complete absence of microorganisms served as control Set exhibited 12% respectively.
关键词：Crude oil;FTIR;Pseudomonas aeruginosa;Pseudomonas stutzeri;Bacillus cereus;Bacillus licheniformis and MSM