文章基本信息

标题：Studies on Isolation and Characterization of Lactase Produced from Soil Bacteria
本地全文：下载
作者：M. Maity ; S. Sanyal ; Bhowal J. 等
期刊名称：Research Journal of Recent Sciences
电子版ISSN：2277-2502
出版年度：2013
卷号：2
期号：8
页码：92-94
语种：English
出版社：International Science Community Association
摘要：The enzyme β-galactosidase has many applications in biotechnology, pharmaceuticals and food processing industries. The present study was conducted to screen the soils from cattle shed near Kolkata for the isolation of lactase producing bacteria. The isolation was performed by plating on lactose agar medium containing the chromogenic substance X-gal. o-nitrophenyl-β-D-galactopyranoside was used as a substrate to determine the β-galactosidase activity of the two isolates BS1 and BS2 which were found to be the active strain producing a considerable amount of β-galactosidase. The bacterial isolate BS1 showed maximum β-galactosidase activity under shaking condition. Both BSI and BS2 produced extracellular and intracellular β-galactosidase activity. The optimum pH and temperature for enzyme activity were 7 and 40°C respectively.
关键词：β-galactosidase;lactose intolerance;o-nitrophenyl-β-D-galactopyranoside;5-bromo-4-chloro-3-idolyl-β-D- galactopyranoside;extracellular enzyme