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标题：Phosphoproteomic Analysis of X enopus laevis Reveals Expression and Phosphorylation of Hypoxia-Inducible PFKFB3 during Dehydration
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作者：Liam J. Hawkins ; Xiaoshuang Wang ; Xiaomin Xue 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2020
卷号：23
期号：10
页码：1-30
DOI：10.1016/j.isci.2020.101598
语种：English
出版社：Elsevier
摘要：SummaryXenopus laevistolerate dehydration when their environments evaporate during summer months. Protein phosphorylation has previously shown to regulate important adaptations inX. laevis, including the transition to anaerobic metabolism. We therefore performed phosphoproteomic analysis ofX. laevisto further elucidate the cellular and metabolic responses to dehydration. Phosphoproteins were enriched in cellular functions and pathways related to glycolysis/gluconeogenesis, the TCA cycle, and protein metabolism, among others. The prominence of phosphoproteins related to glucose metabolism led us to discover that the hypoxia-inducible PFKFB3 enzyme was highly phosphorylated and likely activated during dehydration, a feature of many cancers. Expression of the four transcript variants of thepfkfb3gene was found all to be upregulated during dehydration, potentially due to the enrichment of hypoxia responsive elements in thepfkfb3promoter. These results further support the role of anaerobic glycolysis during dehydration inX. laevisand elucidate a potential mechanism for its increased rate.Graphical AbstractDisplay OmittedHighlights•Clustering analysis indicated a concerted response in liver but not skeletal muscle•Phosphoproteins were enriched for glycolysis, gluconeogenesis, and the TCA cycle•Phosphopeptides of hypoxia-inducible PFKFB3 were highly abundant during dehydration•Four PFKFB3 variants were upregulated potentially supporting anaerobic glycolysisAnimal Physiology; Cell Biology; Proteomics