文章基本信息

标题：Conformational Dynamics of Nonenveloped Circovirus Capsid to the Host Cell Receptor
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作者：Jiarong Li ; Jinyan Gu ; Cui Lin 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2020
卷号：23
期号：10
页码：1-31
DOI：10.1016/j.isci.2020.101547
语种：English
出版社：Elsevier
摘要：SummaryCircovirus, comprising one capsid protein, is the smallest nonenveloped virus and induces lymphopenia. Circovirus can be used to explore the cell adhesion mechanism of nonenveloped viruses. We developed a single-molecule fluorescence resonance energy transfer (smFRET) assay to directly visualize the capsid's conformational feature. The capsid underwent reversible dynamic transformation between three conformations. The cell surface receptor heparan sulfate (HS) altered the dynamic equilibrium of the capsid to the high-FRET state, revealing the HS-binding region. Neutralizing antibodies restricted capsid transition to a low-FRET state, masking the HS-binding domain. The lack of positively charged amino acids in the HS-binding site reduced cell surface affinity and attenuated virus infectivity via conformational changes. These intrinsic characteristics of the capsid suggested that conformational dynamics is critical for the structural changes occurring upon cell surface receptor binding, supporting a dynamics-based mechanism of receptor binding.Graphical AbstractDisplay OmittedHighlights•Circovirus capsid samples have spontaneous and reversible conformations•Receptor binding regulates capsid dynamics•Capsid encountering with receptor induces the exposure of the receptor-binding site•Capsid interaction with the neutralizing antibody can return to the original conformationMolecular Biology; Virology