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标题：Determination of the GC–MS analysis of seed oil and assessment of pharmacokinetics of leaf extract of Camellia sinensis L.
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作者：Saima Rubab ; Ghazala H. Rizwani ; Saraj Bahadur 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2020
卷号：32
期号：7
页码：3138-3144
DOI：10.1016/j.jksus.2020.08.026
语种：English
出版社：Elsevier
摘要：AbstractObjectiveHistory of the utilization of various parts ofCamellia sinensissuch as seed oil in different folklore medicaments and plants leaves for preparing green tea is very old. Pharmacokinetics (PK) plays a key role in the drug discovery stage by channelizing the medicinal chemist in the optimization process of chemical sequences and lends a hand to pharmacologists to invent in vivo studies. The objectives of the present study were to elaborate quantitative determination of phytochemicals and nutritional assessment ofC. sinensisespecially on the composition of seed oil of the localC. sinensisplant and to evaluate the pharmacokinetics of leaves extract in healthy volunteers.MethodDetermination of secondary metabolites and nutritional assessment ofC. sinensiswere carried out. The composition of seed oil was elaborated with the help of GC–MS analysis for its potential significance and evidence-based household uses as folklore but also for industrial applications. Pharmacokinetics of water extract ofC. sinensisleaves was compared in 9 healthy human volunteers and divided into three groups (n = 3, in each) fasting, fed and fed with coke. Plasma concentrations were noted in each group and were quantified using a validated HPLC method.ResultsPhytochemical analysis and proximate analysis ofC. sinensissuggests the presence of alkaloids, flavonoids, tannins, saponins sterols; and protein, lipids fiber and carbohydrate respectively. GC–MS of theC. sinensisseed oil shows the presence of linoleic acid 100%, palmitic acid 33.55%, stearic acid 21.96, caffeine 21.72 and vitamin E 14.49%. Pharmacokinetic studies suggest that in the fasting group, higher plasma concentration was found among all the volunteers.ConclusionThe phytochemical analysis of green tea suggests that it is a good source of secondary metabolites full of important nutrients. GC–MS of seed oil reveals that seed ofC. sinensisis a rich source of fatty acid, and can be used to fulfill the pharmaceutical need. Pharmacokinetic studies may help to develop the biomarkers for the green tea utilization and also would help to design the dose and dose frequency for achieving the significant absorption level. PK and PD also help to determine its plasma concentration and therapeutic efficacy.
关键词：C. sinensis;L.;Phytochemical;Pharmacokinetics;Nutritional assessment