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标题：Heterologous WRKY and NAC transcription factors triggered resistance in Nicotiana benthamiana
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作者：Hafiz Muhammad Khalid Abbas ; Aqeel Ahmad ; Wubei Dong 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2020
卷号：32
期号：7
页码：3005-3013
DOI：10.1016/j.jksus.2020.08.005
语种：English
出版社：Elsevier
摘要：AbstractBackgroundSA (Salicylic acid) and JA (Jasmonic acid)/ET (Ethylene) are the defense and growth hormone regulators involved in alleviating the biotic and abiotic stresses.WRKYandNACgenes are contributors to plant resistance due to their active role in SA and JA/ET defense mechanism.MethodsWRKY and NAC transcription factors (ptHR293andptHR759, respectively) were serially selected (by performing cDNA library functional screening, homology analysis, antioxidant enzymes, ROS burst, callose deposition and qRT-PCR analysis) fromPinellia ternataand transformed intoNicotiana benthamiana. Hybrids were generated to analyze the stability of disease resistance. UPLC-QTOF-MS was performed to study the bioactive compounds.ResultsStudy demonstrated thatptHR293andptHR759had potential to trigger ROS burst and callose deposition inN. benthamianatogether with the activation of PR-genes and antioxidant enzymes. In transformedN. benthamiana, relative expression ofPR-1a(Pathogenesis related-1a) andPDF1.2(Plant defensin 1.2) was upregulated upto 21 folds and 4 folds forptHR293transformedN. benthamiana. WhilePR-1aandPDF1.2exhibited 8 folds and 26 folds upregulation forptHR759transformedN. benthamiana.ptHR293 + ptHR759-F1hybrids also exhibited a significant level of PR-gene expression. Significantly high resistance againstBotrytis cinereawithout influencing the standard seed germination, root and shoot length of transformedN. benthamianawas also observed. A significant induction of bioactive compounds was also observed inptHR293transformedN. benthamiana.ConclusionConclusively, heterologous transcription factors,ptHR293andptHR759, perform their specific role in the activation of SA and JA/ET mediated defense mechanism.
关键词：Hypersensitive response;Nicotiana benthamiana;Transcription factor;WRKY;3,4-Dichloromaleimide