文章基本信息

标题：Aging-Associated Extracellular Vesicles Contain Immune Regulatory microRNAs Alleviating Hyperinflammatory State and Immune Dysfunction in the Elderly
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作者：Hirotake Tsukamoto ; Takahisa Kouwaki ; Hiroyuki Oshiumi 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2020
卷号：23
期号：9
页码：1-37
DOI：10.1016/j.isci.2020.101520
语种：English
出版社：Elsevier
摘要：SummaryAging-associated changes in the immune system often lead to immune dysfunction; however, the mechanisms that underlie this phenomenon have yet to be fully elucidated. This study found that the microRNA-192 (miR-192) is an aging-associated immune regulatory microRNA whose concentration was significantly increased in aged extracellular vesicles (EVs) due to the hyperinflammatory state of aged mice. Interestingly, EV miR-192 exhibited anti-inflammatory effects on macrophages. In our aged mouse model, aging was associated with prolonged inflammation in the lung upon stimulation with inactivated influenza whole virus particles (WVP), whereas EV miR-192 alleviated the prolonged inflammation associated with aging. The hyperinflammatory state of aged mice resulted in reduced production of specific antibodies and efficacy of vaccination with WVP; however, EV miR-192 attenuated this hyperinflammatory state and improved vaccination efficacy in aged mice. Our data indicate that aged EVs constitute a negative feedback loop that alleviates aging-associated immune dysfunction.Graphical AbstractDisplay OmittedHighlights•Extracellular vesicle (EV) miR-192 is an aging-associated microRNA•Hyperinflammatory state in aged mice increases miR-192 levels in EVs•miR-192 within EVs attenuates excessive inflammation in aged mice•EVs containing miR-192 improve vaccination efficacy of aged miceHuman Physiology; Immunology; Molecular Biology; Molecular Physiology