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标题：DR5 is a Suitable System for Studying the Auxin Response in the Poncirus trifoliata-Xanthomonas axonopodis pv. citri Interaction
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作者：Xuan Xiao ; Xiaoyan Li ; Chunli Chen 等
期刊名称：Horticultural Plant Journal
印刷版ISSN：2468-0141
出版年度：2020
卷号：6
期号：5
页码：277-283
DOI：10.1016/j.hpj.2020.07.001
语种：English
出版社：Elsevier
摘要：AbstractThe local auxin distribution characteristics in the roots, stems, and leaves of stably transformed plantlets of trifoliate orange (Poncirus trifoliata) with auxin reporter systemDR5::GUS-YFPwere elucidated in this research. The auxin response maxima could be observed in the apex of the root tip, primary phloem of the tender stem, and the margin of the young leaves according to the activity of theβ-glucuronidase (GUS) reporter gene triggered by the auxin responsiveDR5promoter. Auxin responses in the apex of the root tips increased when treated with synthetic auxin 1-naphthylacetic acid (NAA), but decreased when treated with the auxin polar transportation inhibitor 2,3,5-triiodobenzoic acid (TIBA). These results indicated that theDR5reporter system worked inP. trifoliatafor auxin distribution and response observation. Trifoliate orange is highly susceptible to citrus canker disease. Auxin accumulation was observed visually in the invasion sites of the detached leaves inoculated withXanthomonas axonopodispv.citri(Xac) by GUS staining; the upregulated expression of theYFP, GH3.1, GH3.9, andSAURgenes assessed by quantitative real-time PCR (qRT-PCR) also identified auxin accumulation in the inoculated tissues followingXacinfection. Overall, these findings indicated that the plantlets ofP. trifoliataengineered with the auxin reporter gene provided a promising system for studying auxin responses duringXacinfection.
关键词：KeywordsPoncirus trifoliataDR5auxinXanthomonas axonopodispv.citri