文章基本信息

标题：Basic Fibroblast Growth Factor 2-Induced Proteome Changes Endorse Lewy Body Pathology in Hippocampal Neurons
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作者：Rohit Kumar ; Sainitin Donakonda ; Stephan A. Müller 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2020
卷号：23
期号：8
页码：1-33
DOI：10.1016/j.isci.2020.101349
语种：English
出版社：Elsevier
摘要：SummaryHippocampal Lewy body pathology (LBP) is associated with changes in neurotrophic factor signaling and neuronal energy metabolism. LBP progression is attributed to the aggregation of α-synuclein (α-Syn) and its cell-to-cell transmission via extracellular vehicles (EVs). We recently discovered an enhanced EV release in basic fibroblast growth factor (bFGF)-treated hippocampal neurons. Here, we examined the EV and cell lysate proteome changes in bFGF-treated hippocampal neurons. We identified n = 2,310 differentially expressed proteins (DEPs) induced by bFGF. We applied weighted protein co-expression network analysis (WPCNA) to generate protein modules from DEPs and mapped them to published LBP datasets. This approach revealed n = 532 LBP-linked DEPs comprising key α-Syn-interacting proteins, LBP-associated RNA-binding proteins (RBPs), and neuronal ion channels and receptors that can impact LBP onset and progression. In summary, our deep proteomic analysis affirms the potential influence of bFGF signaling on LBP-related proteome changes and associated molecular interactions.Graphical AbstractDisplay OmittedHighlights•bFGF enhances the neuronal release or extracellular vesicles (EVs)•bFGF induces proteomic changes in the neuronal cell lysate and in neuronal EVs•bFGF incites changes in Lewy body pathology (LBP)-linked protein networks•bFGF-induced molecular interactions may endorse LBPBiological Sciences; Molecular Neuroscience; Proteomics