文章基本信息

标题：Protection by collagen peptides from walleye pollock skin on bone formation via inhibition of oxidative stress
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作者：Feng Liu ; Wencheng Wang ; Yunqiu Xia 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2020
卷号：32
期号：5
页码：2527-2533
DOI：10.1016/j.jksus.2020.03.006
语种：English
出版社：Elsevier
摘要：BackgroundCollagen peptides (CP) have been shown to protect against osteoporosis. Yet, its mechanism on anti-osteoporosis remains unclear.MethodCell viability, levels of alkaline phosphatase (ALP), reactive oxygen species (ROS), malondialdehyde (MDA) and superoxide dismutase (SOD) of hydrogen peroxide (H2O2)-induced oxidative stress in Saos-2 cells pretreated with CP were examined. The effect and regulatory of CP on FBXW7-p100-NF-κB axis and the relationship between the molecules were explored by overexpressing FBXW7, silencing p100 or NF-κB in Saos-2 cells. Western blot and RT-PCR were performed to determine the expressions of FBXW7, p100 and NF-κB. Hematoxylin & Eosin (H&E) staining was performed to determine morphological indexes of bone tissues in ovariectomized-induced osteoporosis rats treated with CP.ResultCP increased cell viability, ALP and antioxidant activities in H2O2-induced oxidative stress in Saos-2 cells. Results on cell viability, ALP, antioxidant levels, protein and gene expression levels in FBXW7-overexpressed, p100-silenced and NF-κB-silenced Saos-2 cells indicate that FBXW7, p100 and NF-κB are the key regulators and CP treatment modulates FBXW7-p100-NF-κB axis in Saos-2 cells. In addition, pretreatment and treatment with CP in ovariectomized rats improved osteoporosis.ConclusionCP could effectively prevent and alleviate osteoporosis. The protective effect of CP on osteoporosis was achieved in part by inhibiting oxidative damage of osteoblasts and promoting bone formation.
关键词：Collagen peptides;Postmenopausal osteoporosis;Oxidative stress;Saos-2 cells;FBXW7