摘要:Camellia tunghinensis chang is an evergreen shrub or small tree of Sect. Chrysantha of Theaceae is endemic to Guangxi and has been listed as a national second-class protected plant. Genetic diversity and mating system of 4 Camellia tunghinensis chang w ild mother and offspring populations (214 individuals in total) were analyzed by SSR m olecular marker. The results show that the genetic diversity of the mother generation and the offspring of the w ild population of Camellia tunghinensis chang is relatively high (the mother population 1=5.250,=0.830,e=0.681,=1.351; the offspring population A/a=6.125,0= 0 .0 .8 4 5 =0.698,/=1.433). M LTR was used to analyze the mating system of parent and offspring of 4 populations (M ZT, MJG, SHL, DLG ) of Camellia tunghinensis chang, and to analyze the parameters of m ultiple sites heterocrossing rate, unit heterocrossing rate and inbreeding number of each population. The results showed that the heterozygosity at m ultiple points and heterozygosity at unit point of 4 populations were relatively high (tm=0.965, t s=0.885). There is a great difference between rt and rpm, which indicates that sub-population structure exists in populations. The outcrossing of the populations is m ainly carried out between in dividuals in the sub-populations, and it is d iffic u lt for the subpopulations to undergo gene flow and d iffe rentiation. The results reveal the mating pattern of Camellia tunghinensis chang, analyze its genetic diversity and genetic differentiation, and provide a prediction for the protection measures and breeding.
关键词:Camellia tunghinensis chang;SSR marker;Genetic diversity;Mating system