文章基本信息

标题：Drosophila PTPMT1 Has a Function in Tracheal Air Filling
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作者：Amanda M. Papakyrikos ; Min Joo Kim ; Xinnan Wang 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2020
卷号：23
期号：7
页码：1-40
DOI：10.1016/j.isci.2020.101285
语种：English
出版社：Elsevier
摘要：SummaryThe fly trachea is the equivalent of the mammalian lung and is a useful model for human respiratory diseases. However, little is known about the molecular mechanisms underlying tracheal air filling during larval development. In this study, we discover that PTPMT1 has a function in tracheal air filling. PTPMT1 is a widely conserved, ubiquitously expressed mitochondrial phosphatase. To reveal PTPMT1's functions in genetically tractable invertebrates and whether those functions are tissue specific, we generate aDrosophilamodel of PTPMT1 depletion. We find that flyPTPMT1mutants show impairments in tracheal air filling and subsequent activation of innate immune responses. On a cellular level, these defects are preceded by aggregation of mitochondria within the tracheal epithelial cells. Our work demonstrates a cell-type-specific role for PTPMT1 in fly tracheal epithelial cells to support air filling and to prevent immune activation. The establishment of this model will facilitate exploration of PTPMT1's physiological functionsin vivo.Graphical AbstractDisplay OmittedHighlights•ADrosophilamodel of PTPMT1 depletion is generated•PTPMT1mutants show impairments in tracheal air filling•Mitochondria aggregate within the tracheal epithelial cells prior to air-filling failure•Depletion of the cardiolipin pathway components does not mimicPTPMT1deficiencyBiological Sciences; Molecular Biology; Cell Biology