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标题：Dynamic Capture and Release of Endoplasmic Reticulum Exit Sites by Golgi Stacks in Arabidopsis
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作者：Junpei Takagi ; Yoshitaka Kimori ; Tomoo Shimada 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2020
卷号：23
期号：7
页码：1-26
DOI：10.1016/j.isci.2020.101265
语种：English
出版社：Elsevier
摘要：SummaryProtein transport from the endoplasmic reticulum (ER) to Golgi stacks is mediated by the coat protein complex COPII, which is assembled at an ER subdomain called ER exit site (ERES). However, the dynamic relationship between ERESs and Golgi stacks is unknown. Here, we propose a dynamic capture-and-release model of ERESs by Golgi stacks inArabidopsis thaliana. Using variable-angle epifluorescence microscopy with high-temporal-resolution imaging, COPII-component-bound ERESs were detected as punctate structures with sizes of 300–500 nm. Some punctate ERESs are distributed on ER tubules and sheet rims, whereas others gather around a Golgi stack in an ER-network cavity to form a beaded-ring structure. Free ERESs that wander into an ER cavity are captured by a Golgi stack in a cytoskeleton-independent manner. Then, they are released by the Golgi stack for recycling. The dynamic ERES cycling might contribute to efficient transfer ofde novosynthesized cargo proteins from the ER to Golgi stacks.Graphical AbstractDisplay OmittedHighlights•VAEM images show dynamic behavior of minimal punctate ERESs•Most of punctate ERESs are distributed on the ER network tubes•Several punctate ERESs contact with a Golgi stack in an ER network cavity•ERESs are dynamically captured and released by Golgi stacksBiological Sciences; Cell Biology; Plant Biology