文章基本信息

标题：BATMAN: Fast and Accurate Integration of Single-Cell RNA-Seq Datasets via Minimum-Weight Matching
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作者：Igor Mandric ; Brian L. Hill ; Malika K. Freund 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2020
卷号：23
期号：6
页码：1-30
DOI：10.1016/j.isci.2020.101185
语种：English
出版社：Elsevier
摘要：SummarySingle-cell RNA-sequencing (scRNA-seq) is a set of technologies used to profile gene expression at the level of individual cells. Although the throughput of scRNA-seq experiments is steadily growing in terms of the number of cells, large datasets are not yet commonly generated owing to prohibitively high costs. Integrating multiple datasets into one can improve power in scRNA-seq experiments, and efficient integration is very important for downstream analyses such as identifying cell-type-specific eQTLs. State-of-the-art scRNA-seq integration methods are based on the mutual nearest neighbor paradigm and fail to both correct for batch effects and maintain the local structure of the datasets. In this paper, we propose a novel scRNA-seq dataset integration method called BATMAN (BATch integration via minimum-weight MAtchiNg). Across multiple simulations and real datasets, we show that our method significantly outperforms state-of-the-art tools with respect to existing metrics for batch effects by up to 80% while retaining cell-to-cell relationships.Graphical AbstractDisplay OmittedHighlights•Current methods for scRNA-seq dataset integration are based on MNN paradigm•MNN paradigm has drawbacks, e.g., it fails in case of non-orthogonal batch effects•BATMAN proposes a new paradigm based on minimum-weight bipartite matching•BATMAN outperforms the existing scRNA-seq integration methods in the gene spaceAlgorithms; Bioinformatics; Transcriptomics