文章基本信息

标题：Hydrogel Glucose Sensor with In Vivo Stable Fluorescence Intensity Relying on Antioxidant Enzymes for Continuous Glucose Monitoring
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作者：Jun Sawayama ; Teru Okitsu ; Akihiro Nakamata 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2020
卷号：23
期号：6
页码：1-21
DOI：10.1016/j.isci.2020.101243
语种：English
出版社：Elsevier
摘要：SummaryHydrogel glucose sensors with boronic acid-based fluorescence intensity theoretically hold promise to improvein vivocontinuous glucose monitoring (CGM) by facilitating long-lasting accuracy. However, these sensors generally degrade after implantation and the fluorescence intensity decreases immediately over time. Herein, we describe a hydrogel glucose sensor within vivostability based on boronic acid-based fluorescence intensity, integrating two antioxidant enzymes, superoxide dismutase (SOD), and catalase. These protected the arylboronic acid from being degraded by hydrogen peroxidein vitroand preserved the boronic acid-based fluorescence intensity of the hydrogel glucose sensors in rats for 28 days. These antioxidant enzymes also allowed the hydrogel glucose sensor attached to a homemade semi-implantable CGM device to trace blood glucose concentrations in rats for 5 h with the accuracy required for clinical settings. Hydrogel glucose sensors with boronic acid-based fluorescence intensity containing SOD and catalase could comprise a new strategy forin vivoCGM.Graphical AbstractDisplay OmittedHighlights•The arylboronic acids of hydrogel glucose sensors are sensitive to cleavage by ROS•The antioxidant enzymes suppress the degradation of fluorescence effectivelyin vivo•The developed sensor performs CGM with the accuracy required for clinical settingsBiochemical Mechanism; Glycobiology; Medical Device; Sensor