文章基本信息

标题：A novel use of the leukocyte coping capacity assay to assess the immunomodulatory effects of organohalogenated contaminants in avian wildlife
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作者：Elisabeth Hansen ; Nikolaus Huber ; Jan O. Bustnes 等
期刊名称：Environment International
印刷版ISSN：0160-4120
电子版ISSN：1873-6750
出版年度：2020
卷号：142
页码：1-8
DOI：10.1016/j.envint.2020.105861
语种：English
出版社：Pergamon
摘要：Highlights•White-tailed eagle chicks show high levels of organohalogenated contaminants (OHCs).•Leukocyte Coping CapacityLCCis a functional real-time measure of innate immunity.•Higher OHC exposure resulted in a reducedLCC,regardless of OHC concentrations.•Our results suggest immunomodulatory effects of both legacy and emerging OHCs.•We proposeLCCas a relevant assay to expand the toolbox of wildlife ecotoxicology.AbstractApex predators are characterized by high levels of biomagnifying organohalogenated contaminants (OHCs) which have been found to induce detrimental health effects in wildlife, such as immune system impairment. The leukocyte coping capacity (LCC) assay is a functional real-time measure of an innate immune response essential in pathogen resistance, known as the respiratory burst. The current study suggests the novel use of this tool to test whether OHCs impair the innate immune system of a sentinel top predator, the white-tailed eagle (Haliaeetus albicilla; WTE). The LCC analysis was performed in the field on WTE nestlings (n = 84) from northern Norway over two breeding seasons. Poly- and perfluoroalkyl substances (PFAS) dominated the total OHC load, surpassing the levels of legacy organochlorines. In addition, we detected significant negative correlations between concentrations of all polychlorinated biphenyls,p,p′-dichlorodiphenyldichloroethylene, perfluorohexane sulfonic acid and long-chain perfluorocarboxylic acids and the LCC of WTE nestlings. Based on our current findings reflecting a potential negative effect of both emerging and legacy OHCs on innate immune capacity, we suggest LCC to be a relevant and accessible test expanding the ecotoxicological toolbox to assess sub-lethal effects of OHCs in apex avian wildlife.
关键词：Bird of prey;Innate immune system;Organochlorines;Poly- and perfluoroalkyl substances;Sub-lethal effects;White-tailed eagle