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标题：The Investigation on Cholinesterases and BACE1 Inhibitory Activities in Various Tea Infusions
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作者：Uthaiwan SUTTISANSANEE ; Thanit KUNKEAW ; Natthaphon THATSANASUWAN 等
期刊名称：Walailak Journal of Science and Technology (WJST)
印刷版ISSN：2228-835X
出版年度：2019
卷号：16
期号：3
页码：165-174
语种：English
出版社：Institute of Research and Development, Walailak University.
摘要：Alzheimer’s disease (AD) is a chronic neurological disease related to the decline in brain and nervous system functions. At present, inhibitions of cholinesterases (ChEs) including acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) to enhance neurotransmitters and retardation of b-amyloid formation through b-secretase (BACE1) inhibition are 2 hypotheses for AD prevention and treatment. Hence, this study aims to investigate inhibitory activities against ChEs of various tea varieties of Camellia sinensis and herbal teas. At this step, the screening methodology for ChEs inhibitory activities in various tea varieties in order to provide a fast, inexpensive and convenient method for the detection of anti-cholinesterase activity was optimized. Then, selected tea with high ChEs was further examined regarding its BACE1 activity. As results, all tea samples displayed both anti-AChE and -BChE activities with the broad ranges of 6 - 85 % and 0 - 72 % inhibitory activities, respectively. Interestingly, C. sinensis teas exhibited higher ChEs inhibitory activities than herbal teas, in exception of Pandanus amaryllifolius (pandan) tea, which exhibited comparable inhibitory activities as C. sinensis teas. Pandan leaves were found to exhibit anti-BACE1 activity with 98 % inhibition. Besides, preliminary in vivo study showed that pandan leaves extract was able to rescue climbing defect observed in AD Drosophila melanogaster model, indicating the potential roles of pandan leaves in AD prevention.DownloadsDownload data is not yet available.ReferencesA Tripathi and UC Srivastava. Acetylcholinesterase: A versatile enzyme of nervous system. Ann. Neurosci. 2008; 15, 106-110.
关键词：Alzheimer’s disease;anti-cholinesterase activities;assay conditions;Drosophila melanogaster;tea infusion