文章基本信息

标题：DNA Dumbbell and Chameleon Silver Nanoclusters for miRNA Logic Operations
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作者：Yiting Jiang ; Peng Miao
期刊名称：Research
电子版ISSN：2639-5274
出版年度：2020
卷号：2020
页码：1-8
DOI：10.34133/2020/1091605
语种：English
出版社：American Association for the Advancement of Science
摘要：Multiplex miRNA analysis is a fundamental issue for exploring a complex biological system and early diagnosis of miRNA-related diseases. Herein, we have developed a series of novel logic gates for miRNA analysis coupling DNA nanostructures and chameleon silver nanoclusters (AgNCs). DNA dumbbell structures are firstly designed with two independent nucleation sequences for AgNCs at the 5 and 3 ends, respectively. By introducing different miRNA inputs, separations of two AgNCs are controlled and the fluorescence property of AgNCs changes. By studying the ratiometric fluorescence responses, sensitive and selective analysis of multiple miRNAs can be achieved. The present work provides powerful tools for miRNA diagnostics and may also guide future DNA nanostructure-based logic gates.