文章基本信息

标题：COVALENT CONJUGATION AND CHARACTERIZATION OF THE EBOLA NUCLEOPROTEIN 421-430 PEPTIDE WITH POLYACRYLIC ACID
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作者：Tayfun Acar ; Burcu Ucar ; Pelin Pelit Arayici 等
期刊名称：Fresenius Environmental Bulletin
印刷版ISSN：1018-4619
出版年度：2020
卷号：29
期号：7
页码：5214-5219
语种：English
出版社：PSP Publishing
摘要：The main purpose of this study is the synthesis and characterization of the bioconjugates of poly-aciylic acid and the Ebola virus synthetic peptide (421-430 sequence in virus nuclcoprotcin). The peptide-polymer bioconjugates were synthesized at various conjugation ratios with changing the peptide concentrations. The physicochemical characterization of the obtained bioconjugates was per-formed using Fluorescence Spectroscopy and Size Exclusion Chromatography. The optimum conjugation ratio was determined by considering the amount of umeacted peptide calculated by evaluating the two analysis methods. The obtained results showed that the conjugates were synthesized in average 50% reaction efficiency and the fluorescence intensity decreased in every conjugation ratio relative to the fluorescence intensity of the equivalent amount of peptide. The results indicate the conjugation reaction was performed successfully and the 丫（npepudo/npAA ratio) = 9 conjugate was found the optimum conjugation ratio for (his peptide and polymer. It is believed (hat the obtained results can be used as an antigenic peptide vaccine model against the Ebola virus in future studies.
关键词：Ebola virus disease;Synthetic peptide;Polyacrylic acid;Conjugation