标题:EFFECT OF STORAGE TEMPERATURE AND DURATION IN PUPAL STAGE ON EMERGENCE AND SOME BIOLOGICAL PARAMETERS OF H ELICOVERPA ARM IGERA (HUBNER) (LEPIDOPTERA: NOCTUIDAE)
摘要:The main aim of this study is to determine the effects of various storage temperatures (5, 10 and 150C) and durations (15, 30, 45, 60, 75 and 90 days) on some biological properties (adult emergence ratio, adult emergence time after low-temperature storage, adult longevity, ovipositing female ratio, pre- oviposition, oviposition and post-oviposition periods, daily oviposition performance and fecundity) of Helicoverpa armigera (Hubner) (Lep.: Noctuidae) pupae. The study was carried out between 2017 and 2018 in the laboratory. OVeiipaveim (Hubner) larvae were reared on artificial insect diet in a climate chamber (25士 10C, 60士5% relative hum idity and L16:D8). Newly formed pupae were used in the low-temperature storage. After the low-temperature storage process completed, .arm igera pupae were kept in the climate chamber. Experiments were carried out in a completely randomized design at 3 replicates that contain 15 pairs of H.armigera pupae. Results show that the emergence ratio, adult longevity, oviposition female ratio, oviposition period and fecundity decrease as cold storage duration increased. The time period from low-temperature storage to adult emergence decreased after various storage durations at 10 and 150C whereas increased at 50C. Also, females always emerged before males at any storage duration and temperature. After low-temperature storage of H.armigera pupae for 15 days at 5, 10 and 150C and 30 days at 5 and 100C, adult emergence ratio, adult emergence time after low-temperature storage, adult longevity, ovipositing female ratio, pre-oviposition, oviposition and post-oviposition periods, oviposition rate and fecundity was similar to control experiment. When H.armigera is used as the host for parasitoid species, the pupae may be stored at 10 or 150C temperature for up to 30 days when the parasitoid is not needed in seasons such as winter. These results may be useful in biological control programs or IPM programs.