文章基本信息

标题：ANTIOXIDANT RESPONSES IN THE CYTOTOXICITY OF ISOLATED RAINBOW TROUT {ONCORHYNCUS M YKISS) HEPATOCYTES INDUCED BY DI(2-ETHYLHEXYL) PHTHALATE
本地全文：下载
作者：Gurbet Ceylan Kaptaner ; Bedia Bati ; Burak Kaptaner 等
期刊名称：Fresenius Environmental Bulletin
印刷版ISSN：1018-4619
出版年度：2020
卷号：29
期号：6
页码：4364-4371
语种：English
出版社：PSP Publishing
摘要：Di(2-ethylhexyl) phthalate (DEHP) is a plasticizer that is widely used. In the present study, the primary cultured hepatocytes of rainbow trout (O n- corhyncus my kiss) were exposed to DEHP (0,25, 50, 100, and 200 M ) for 24 h and the cytotoxic effects were investigated using the lactate dehydrogenase (LDH) release and antioxidant defense system biomarkers. According to the results, DEHP caused significant LDH releases at concentrations of 50, 100, and 200 M . The superoxide dismutase (SOD) activity increased significantly with 25 M DEHP, whereas its activity was significantly decreased with 100 M DEHP. The catalase (CAT) activity significantly increased with all of the DEHP concentrations, while a simultaneous decrease in CAT activity with SOD activity was observed with 100 M DEHP. The glutathione peroxidase activity increased with all of the DEHP concentrations. The glutathione - S -transferase activity remained unchanged with 25, 50, and 100 M DEHP, but increased significantly with 200 M DEHP. Levels of reduced glutathione elevated with 50 and 200 M DEHP. The results suggested that DEHP cytotoxicity is involved in antioxidant responses in hepatocytes. The study will offer insight into the mechanism of DEHP-induced cytotoxicity.
关键词：Di(2-ethylhexyl) phthalate;rainbow trout;Oncorhyncus mykiss;cytotoxicity;antioxidant defense