文章基本信息

标题：Reliable and Efficient Agrobacterium tumefaciens-Mediated Genetic Transformation of Dianthus spiculifolius
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作者：Jingang Wang ; Shiyue Liu ; Hongping Ma 等
期刊名称：Horticultural Plant Journal
印刷版ISSN：2468-0141
出版年度：2020
卷号：6
期号：3
页码：199-204
DOI：10.1016/j.hpj.2020.03.004
语种：English
出版社：Elsevier
摘要：AbstractDianthus spiculifoliusis a perennial herbaceous flower with strong environmental adaptability and is an important ornamental ground cover plant. In this study, seeds ofD. spiculifoliuswere used as explants for callus induction, adventitious bud differentiation, and rooting by adding different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzyl aminopurine (6-BA), and naphthaleneacetic acid (NAA) to Murashige and Skoog medium. The calli generated were co-cultured withAgrobacterium tumefaciensEHA105 containing pBI121-GUS or pBI121-GFP plasmids for 30 min, and transgenic regenerated plants were obtained by kanamycin (30 mg · L−1) screening. RT-PCR confirmed the stable expression of the exogenousGUSandGFPgenes in theD. spiculifolius. Theβ-glucuronidase (GUS) histochemical staining confirmedGUSgene expression in transgenic calli, adventitious buds, and regenerated plants ofD. spiculifolius. The green fluorescent protein (GFP) visual analysis showedGFPgene expression in transgenic calli. Furthermore, subcellular localization analysis showed that the three organelle marker proteins were not only successfully expressed but also accurately localized to their corresponding organelles inD. spiculifoliuscallus cells. These results indicated a successful establishment of a reliable and efficientA. tumefaciens-mediated genetic transformation system, which will contribute to functional gene research and genetic improvement ofD. spiculifolius.
关键词：KeywordsAgrobacterium tumefaciensDianthus spiculifoliusCallusTissue culturePlant transformation