文章基本信息

标题：Injectable Therapeutic Organoids Using Sacrificial Hydrogels
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作者：Ninna S. Rossen ; Priya N. Anandakumaran ; Rafael zur Nieden 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2020
卷号：23
期号：5
页码：1-38
DOI：10.1016/j.isci.2020.101052
语种：English
出版社：Elsevier
摘要：SummaryOrganoids are becoming widespread in drug-screening technologies but have been used sparingly for cell therapy as current approaches for producing self-organized cell clusters lack scalability or reproducibility in size and cellular organization. We introduce a method of using hydrogels as sacrificial scaffolds, which allow cells to form self-organized clusters followed by gentle release, resulting in highly reproducible multicellular structures on a large scale. We demonstrated this strategy for endothelial cells and mesenchymal stem cells to self-organize into blood-vessel units, which were injected into mice, and rapidly formed perfusing vasculature. Moreover, in a mouse model of peripheral artery disease, intramuscular injections of blood-vessel units resulted in rapid restoration of vascular perfusion within seven days. As cell therapy transforms into a new class of therapeutic modality, this simple method—by making use of the dynamic nature of hydrogels—could offer high yields of self-organized multicellular aggregates with reproducible sizes and cellular architectures.Graphical AbstractDisplay OmittedHighlights•Therapeutic, prevascularized organoids were formed in a sacrificial scaffold•The organoids are highly reproducible and grown in a high-throughput manner•The organoids rapidly formed perfusing vasculature in healthy mice•Therapeutic potential was assessed in a mouse model of peripheral artery diseaseBiotechnology; Bioelectronics; Biomaterials