文章基本信息

标题：Microinjection support system for small biological subjects
本地全文：下载
作者：Yasuhiro Sugimoto ; Keisuke Naniwa ; Hitoshi Aonuma 等
期刊名称：OpenHardwareX
印刷版ISSN：2468-0672
出版年度：2020
卷号：7
页码：1-15
DOI：10.1016/j.ohx.2020.e00103
语种：English
出版社：Elsevier
摘要：Graphical abstractDisplay OmittedAbstractIn biological research, various experiments such as behavioral experiments and physiological ones are often conducted with pharmacologically treated animals. In such experiments, it is necessary to inject the same volume of solution into numerous small animals, such as insects to prepare several experimental subjects. However, repeating manual injections is burdensome, and it is also difficult to maintain injection quality and consistency. We have developed a microinjection system that can support and semiautomate the injections of small animals. The system consists of two cameras, a micromanipulator, a syringe pump, and a structural framework all operated from a personal computer to quickly inject the same volume of liquid solutions at the same position and depth into small animals. The microinjection system has sufficient extensibility for it to be used in a variety of applications.
关键词：Injection system;Liquid solution injection;Sample preparation;Open source robotics;Image processing