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标题：Compatibility of Site-Specific Recombination Units between Mobile Genetic Elements
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作者：Shota Suzuki ; Miki Yoshikawa ; Daisuke Imamura 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2020
卷号：23
期号：1
页码：1-35
DOI：10.1016/j.isci.2019.100805
语种：English
出版社：Elsevier
摘要：SummarySite-specific recombination (SSR) systems are employed for transfer of mobile genetic elements (MGEs), such as lysogenic phages and integrative conjugative elements (ICEs). SSR betweenattP/IandattBsites is mediated by an integrase (Int) and a recombination directionality factor (RDF). The genome ofBacillus subtilis168 contains SPβ, an active prophage,skin, a defective prophage, and ICEBs1, an integrative conjugative element. Each of these MGEs harbors the classic SSR unitattL-int-rdf-attR. Here, we demonstrate that these SSR units are all compatible and can substitute for one another. Specifically, when SPβ is turned into a defective prophage by deletion of its SSR unit, introduction of the SSR unit ofskinor ICE converts it back to an active prophage. We also identified closely related prophages with distinct SSR units that control developmentally regulated gene rearrangements ofkamA(L-lysine 2,3-aminomutase). These results suggest that SSR units are interchangeable components of MGEs.Graphical AbstractDisplay OmittedHighlights•Lysogenic phage-derived SSR unit is sufficient to drive SSR of ICE and vice versa•Defective prophage-derived SSR unit can drive the excision of the active lysogenic phage•Closely related prophages with distinct SSR units control each gene rearrangements•Correspondence between MGEs and their cognate SSR units is not absoluteGenetics; Molecular Genetics; Microbiology; Microbial Genetics