文章基本信息

标题：Myosin-18B Promotes Mechanosensitive CaMKK2-AMPK-VASP Regulation of Contractile Actin Stress Fibers
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作者：Shuangshuang Zhao ; Xuemeng Shi ; Yue Zhang 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2020
卷号：23
期号：4
页码：1-24
DOI：10.1016/j.isci.2020.100975
语种：English
出版社：Elsevier
摘要：SummaryActin stress fibers guide cell migration and morphogenesis. During centripetal flow, actin transverse arcs fuse accompanied by the formation of myosin II stacks to generate mechanosensitive actomyosin bundles. However, whether myosin II stack formation plays a role in cell mechano-sensing has remained elusive. Myosin-18B is a “glue” molecule for assembling myosin II stacks. By examining actin networks and traction forces, we find that cells abolishing myosin-18B resemble Ca2+∕calmodulin-dependent kinase kinase 2 (CaMKK2)-defective cells. Inhibition of CaMKK2 activity reverses the strong actin network to thin filaments in myosin-18B-overexpressing cells. Moreover, AMP-activated protein kinase (AMPK) activation is able to relieve the thin stress fibers by myosin-18B knockout. Importantly, lack of myosin-18B compromises AMPK-vasodilator-stimulated phosphoprotein and RhoA-myosin signaling, thereby leading to defective persistent migration, which can be rescued only by full-length and C-extension-less myosin-18B. Together, these results reveal a critical role of myosin-18B in the mechanosensitive regulation of migrating cells.Graphical AbstractDisplay OmittedHighlights•Myosin-18B knockout cells resemble cells dampening mechano-sensing signaling pathway•Myosin-18B depletion decreases the phosphorylation level of AMPK-VASP and MLC•Myosin-18B knockout cells show compromised persistent migration•The N-extension and coiled-coil domain of myosin-18B is indispensable in cell migrationBiological Sciences; Cell Biology; Functional Aspects of Cell Biology