文章基本信息

标题：Corneal ulcer/keratitis derived Aspergillus flavus & Aspergillus tamarii and their RAPD-PCR typing
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作者：Bader Alshehri ; Palanisamy Manikandan ; Ahmed Abdel-hadi 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2020
卷号：32
期号：3
页码：2103-2111
DOI：10.1016/j.jksus.2020.02.019
语种：English
出版社：Elsevier
摘要：AbstractThe aim of the study was investigate the DNA polymorphisms ofAspergillus flavusandAspergillus tamariiisolated from regional fungal keratitis cases based on random amplified polymorphic DNA (RAPD) analyses. Thirty-twoA. flavusand sixA. tamariiisolates were identified using conventional macroscopic and microscopic features. Five decamer primers were used to amplify the genomic DNA sequences of the test isolates, and 140 total RAPD patterns were generated among them. From each of the 38 isolates, a range of 15–30 RAPD types were revealed. All of these were isolated from male farmers from Tamil Nadu. In the future, RAPD analyses could be employed as a reliable and rapid technique to differentiate among keratitis isolates of fungal pathogens.
关键词：RAPD PCR;Eye infection;Aspergillus flavus;Corneal ulcer;Molecular typing;Keratitis