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标题：Anticandidal and anti-carcinogenic activities of Mentha longifolia (Wild Mint) extracts in vitro
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作者：Mohamed Taha Yassin ; Ashraf A. Mostafa ; Abdulaziz A. Al-Askar 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2020
卷号：32
期号：3
页码：2046-2052
DOI：10.1016/j.jksus.2020.02.008
语种：English
出版社：Elsevier
摘要：AbstractVulvovaginal candidiasis is a common health problem affecting about 75% of women at reproductive age. Teratogenicity and fetal toxicity resulting from antifungal therapy during pregnancy necessitates formulation of novel therapeutic agents. Disk diffusion method was performed to evaluate the antifungal activity ofMentha longifoliaextracts against different candidal strains. Moreover, the anticancer activity of differentM. longifoliaextracts against MCF7 breast cancer cell line was evaluated by MTT assay. TheM. longifolian-hexane extract exhibited the highest antifungal activity, while the methanolic extract showed no antimicrobial activity against the testedCandidastrains. Minimum inhibitory concentration (MIC) of n-hexane extract was 250 µg/disk againstC. tropicaliswhile it was 500 µg/disk againstC. albicansandC. glabrata. Minimum fungicidal concentration (MFC) was 0.5 mg/disk againstC. albicansandC. tropicalis,and 1 mg/disk againstC. glabrata. GC–MS analysis of the n-hexane extract was performed to detect the active phytochemical components. Menthone (18.37%) was the main phytochemical active component of n-hexane extract followed by butyloctanol (16.13%), 1,5-(1-Bromo-1-methylethyl)-2-methyl-2-cyclohexen-1-one (14.89%), α-tocopherol (13.13%), eugenol (12.21%), α-resorcylamide (11.56%), citronellal (3.71%), butylated hydroxytoluene (3.21%), isocaryophyllene (2.81%), 2-hexyldecanol (2.12%) and tau-cadinol (1.87%). The methanolic extract exhibited the highest anticancer activity against MCF7 breast cancer cell line while diethyl ether extract exhibited the lowest activity with IC50of 91.67 and 244.70 µg/ml respectively.M. longifoliaextracts could be used as a potential source of novel anticandidal and anti-carcinogenic therapeutic agents.
关键词：Vulvovaginal candidiasis;Mentha longifolia;Anti-candida;Minimum inhibitory concentration;Cytotoxicity;Phytochemicals