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标题：Antimicrobial Resistance Gene Prevalence in a Population of Patients with Advanced Dementia Is Related to Specific Pathobionts
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作者：Aislinn D. Rowan-Nash ; Rafael Araos ; Erika M.C. D'Agata 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2020
卷号：23
期号：3
页码：1-33
DOI：10.1016/j.isci.2020.100905
语种：English
出版社：Elsevier
摘要：SummaryLong-term care facilities are significant reservoirs of antimicrobial-resistant organisms, and patients with advanced dementia are particularly vulnerable to multidrug-resistant organism (MDRO) acquisition and antimicrobial overuse. In this study, we longitudinally examined a group of patients with advanced dementia using metagenomic sequencing. We found significant inter- and intra-subject heterogeneity in microbiota composition, suggesting temporal instability. We also observed a link between the antimicrobial resistance gene density in a sample and the relative abundances of several pathobionts, particularlyEscherichia coli,Proteus mirabilis, andEnterococcus faecalis, and used this relationship to predict resistance gene density in samples from additional subjects. Furthermore, we used metagenomic assembly to demonstrate that these pathobionts had higher resistance gene content than many gut commensals. Given the frequency and abundances at which these pathobionts were found in this population and the underlying vulnerability to MDRO of patients with advanced dementia, attention to microbial blooms of these species may be warranted.Graphical AbstractDisplay OmittedHighlights•Longitudinal analysis of the rectal microbiota of patients with advanced dementia•The microbiota was temporally unstable and characterized by pathobiont blooms•Antimicrobial resistance gene burden correlated with abundances of pathobionts•Genome assembly revealed that these species carried high levels of resistance genesGenomics; Microbiome; Multi-Drug Resistant Organisms