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标题：Effect of Danggui-Shaoyao-San on renal macrophages in STZ-induced DN rats
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作者：Xiaobing Li ; Xinyan Miao ; Liang Wang 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2020
卷号：32
期号：2
页码：1778-1784
DOI：10.1016/j.jksus.2020.01.016
语种：English
出版社：Elsevier
摘要：AbstractObjectives:The present study is to explore the influence of DSS on the proliferation and activation of renal macrophages in the STZ-induced diabetic nephropathy rats.Methods:Seventy rats were grouped according to the random digit table means. 8 rats were randomly selected as the control group. The remaining 62 rats were divided into 5 groups and given a high-fat and high-sugar diet for 4 weeks. Diabetic rats were prepared by intraperitoneal injection of 55 mg/kg STZ. Rats in the DSS groups were given 21 g/kg/day, 14 g/kg/day and 7 g/kg/day DSS separately. The rats in the positive control group were given 6 mg/kg/day TP, while the rats of the model group and control group were received the physiological saline by gastric perfusion directly. Blood glucose, 24 h urine protein, urine urea nitrogen and β2 microglobulin of the rats were measured. And the protein levels of ED-1, MCP-1 and TLR4 in rat kidney were detected by Western Blot. ED-1+/TLR4+, ED-1+/PCNA+ and ED-1+/iNOS+ were detected by immunofluorescence.Results:DSS reduced the levels of blood glucose, 24-h urine protein, urine urea nitrogen and β2 microglobulin and repaired kidney tissue damage of the DN rats. In the WB experiment, DSS clearly decreased the protein expression levels of ED-1, MCP-1, and TLR4 in a dose-dependent manner (p < 0.05). In the immunofluorescence experiment, compared with the model group, the level of ED-1+/TLR4+ in the rats of DSS and TP groups and the level of ED-1+/PCNA+ in the rats of DSS-M group were obviously decreased (p < 0.05). Also, the level of ED-1+/PCNA+ and ED-1+/iNOS+ in the rats of DSS-H and TP groups was decreased evidently (p < 0.01).Conclusion:We inferred that DSS has therapeutic effects on the DN model rats, and its mechanism is likely to be connected with inhibition of macrophage proliferation and activation in rat kidney.
关键词：Diabetic Nephropathy;Rats;Macrophage;Activation;Danggui-Shaoyao-San