文章基本信息

标题：Tooth bioengineering from single cell suspensions
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作者：Vassilis Stratoulias ; Frederic Michon
期刊名称：MethodsX
印刷版ISSN：2215-0161
电子版ISSN：2215-0161
出版年度：2019
卷号：6
页码：2429-2438
DOI：10.1016/j.mex.2019.10.009
语种：English
出版社：Elsevier
摘要：Graphical abstractDisplay OmittedAbstractRecent advances in bioengineering and biomaterials, along with knowledge deriving from the fields of developmental biology and stem cell research, have rendered feasible functional replacement of full organs. Here, we describe the methodology for bioengineering a tooth, starting from embryonic epithelial and mesenchymal single cell suspensions. In addition, we describe the subsequent steps of processing this minute structure for use in applications such as histological examination, immunofluorescence andin situhybridisation. This methodology can be used for any minute structure that needs to be used in paraffin blocks.•Detailed methodology for reproducible and reliable results•Extra step to ensure single cell populations•Subsequent minute structure processing for histological analysis
关键词：Tooth;Bioengineering;Reconstruction;Dental implant;Epithelium;Mesenchyme