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标题：Detection of helminth ova genera using in-situ biosynthesis of gold nanoparticles
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作者：Vivek B. Ravindran ; Adam Truskewycz ; Andrew S. Ball 等
期刊名称：MethodsX
印刷版ISSN：2215-0161
电子版ISSN：2215-0161
出版年度：2019
卷号：6
页码：993-997
DOI：10.1016/j.mex.2019.04.026
语种：English
出版社：Elsevier
摘要：Graphical abstractDisplay OmittedAbstractIn this study, a presumptive colorimetric method was used to detect and differentiate the ova of two major soil transmitted helminths in wastewater,AscarisandTrichuris. Gold nanoparticles were synthesised following the reduction of tetrachloroauric acid by the surface moiety ofAscaris suum, resulting in a colour change. In contrast there was no colour change withTrichuris suisindicating the absence of gold nanoparticle synthesis. Analysis of the ova using scanning electron microscopy (SEM) revealed that the synthesis of nanoparticles on the surface of ova was confirmed as gold nanoparticles (91 w/w %) by energy dispersive X-ray analysis (EDX). This study indicated that the surface moieties of helminth ova could be a potential target for ova detection and differentiation using the biosynthesis of nanoparticles by colorimetry methods.Three advantages:•Simple colorimetry based method requiring no sophisticated devices.•No trained personnel required.•Cost-effective and can be a potential candidate for biosensors.
关键词：Ascaris;Trichuris;Wastewater;Helminth ova;Gold nanoparticles;SEM;EDX