文章基本信息

标题：Azadirachta indica based biosynthesis of silver nanoparticles and evaluation of their antibacterial and cytotoxic effects
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作者：M. Asimuddin ; Mohammed Rafi Shaik ; Syed Farooq Adil 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2020
卷号：32
期号：1
页码：648-656
DOI：10.1016/j.jksus.2018.09.014
语种：English
出版社：Elsevier
摘要：AbstractDue to the growing threat to the environment by the climate change, the development of sustainable and environmental friendly methods for the preparation of metallic nanoparticles (NPs) is highly desirable. Herein, we demonstrate a simple and eco-friendly method for the preparation of silver nanoparticles (AgNPs) usingAzadirachta indicaleaf extract and aqueous solution of silver nitrate (AgNO3). The effect of concentration of leaf extract and temperature on the synthesis of AgNPs is studied. It was revealed that 1 mM of AgNO3, leaf extract concentration of 60 mg/mL, 30 min of reaction time and a temperature 85 °C were found to be optimum for the synthesis good quality AgNPs. The formation of AgNPs was confirmed by UV–Vis analysis, where the UV spectrum exhibited a highest absorption peak at ∼410 nm corresponding to the surface Plasmon Resonance (SPR) of the AgNPs. Furthermore, the antibacterial activity of biosynthesised AgNPs was investigated and the inhibitory concentrations of 0.5 µg/mL forEscherichia coliand 1.0 µg/mL forStaphylococcus aureuswere found. Besides, the in vitro cytotoxicity investigation of biosynthesized AgNPs against humanacute lymphoblastic leukemiacells via MTT assay showed that the minimum inhibitory concentration of 15.6 µg/mL was required. Therefore, the eco-friendly and cost effective synthesis of AgNPs has potential for biomedical applications and this method can be used for the large scale production of AgNPs.
关键词：Azadirachta indica;AgNPs;Biosynthesis;Antimicrobial activities;Cytotoxicity