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标题：Parturition affects test-positivity in sheep with subclinical paratuberculosis; investigation following a preliminary analysis
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作者：Antonia Mataragka ; Kyriaki Sotirakoglou ; Maria Gazouli 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2019
卷号：31
期号：4
页码：1399-1403
DOI：10.1016/j.jksus.2019.02.009
语种：English
出版社：Elsevier
摘要：AbstractWithin the context of an investigation focused at improving the effectiveness of test-and-removal for the control of ovine paratuberculosis, we recently conducted a preliminary study using ELISA and real time PCR to assess whether parturition affects test-positivity, in animals with subclinical paratuberculosis. Samples of faeces and blood were collected from 42 adult female animals, before (PP1) and after parturition (PP2), and before mating (PP3). In the preliminary stage of the analysis, only one of the animals tested reacted positively to ELISA (2.38%, 1 of 42), which corresponds to 2.8% of PCR-reactors (1 of 36). Therefore, the final stage the investigation was conducted using only real time PCR, which was applied to test samples of faeces collected from 85 animals, in 5 periods of sampling: 4–15/1–3 days (FP1/FP2) before and after parturition (FP3/FP4), and before mating (FP5). The result of the preliminary analysis indicated that PCR-positivity in terms of the number of shedders and the amount of MAP, is statistically significantly lower before parturition (PP1), whereas that of the final, higher in FP4 compared to FP5. Significantly higher levels of positivity in PP2 and FP4 were also recorded in connection with animals reacting positively to PCR more than once. In conclusion, in sheep with subclinical paratuberculosis, the period of 4–15 days postpartum is more suitable for the application of test-and-removal aiming to the control of the disease, using real time PCR. The use of ELISA for the same purpose is not recommended in the specific category of animals, due to low sensitivity.
关键词：Paratuberculosis;Parturition;Shedding of MAP;Real time PCR;ELISA