文章基本信息

标题：THE EFFECTS OF THYME ESSENTIAL OIL ON ENZYME ACTIVITIES OF RHIZOCTONIA SOLANI
本地全文：下载
作者：Hulya Ozgonen Ozkaya ; Osman Onat
期刊名称：Fresenius Environmental Bulletin
印刷版ISSN：1018-4619
出版年度：2019
卷号：28
期号：12
页码：9560-9567
语种：English
出版社：PSP Publishing
摘要：In this study, determination of the effects of different concentrations of essential oil of Origanum onites L. (Izmir Thyme) on mycelial development of Rhizoctonia solani in liquid medium, total protein amount, pectin-methyl-esterase and cellulase activity produced in culture filtrate and mycelial extract were aimed. In the study, spectrophotometric analyzes were carried out to determine the substances of protein structure. As a result of HPLC analysis, carvacrol and thymol were detected at higher rate in thyme oil used. Thyme oil reduced mycelial biomass of R. solani at increasing concentrations (20-100%) compared to the control and completely inhibited at 1000 ppm concentration. Analysis of the culture filtrate and mycelial extracts obtained from the development of R. solani under certain conditions in the thyme oil added media resulted in a reduction in the total protein content. Thyme oil reduced pectin-methyl-esterase activity of R. solani at increasing concentrations. Mycelial development of R. solani was inhibited at 1000 ppm concentration but the Pectin- methyl-esterase activity was stopped at 750 ppm of thyme oil. Thyme oil reduced cellulase activity at increasing concentrations and stopped completely at 1000 ppm concentration. The effects of thyme oil on the enzyme activities of R. solani were found to be caused by the higher rates of carvacrol and tymol. Our study has promising results in terms of thyme oil, which is known to be effective against R. solani, and to provide a more detailed explanation of the efficacy of different concentrations on the pathogen control and more conscious use.
关键词：Thyme;Rhizoctonia solani;Carvacrol;Total protein;Pectin methyl esterase;Cellulase