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  • 标题:RESISTANT MECHANISM OF TAUSCH9S GOATGRASS (AEGILOPS TAUSCHII COSSON) TO MESOSULFURON-METHYL
  • 本地全文:下载
  • 作者:Junjie Liu ; Xu Su ; Yashe Li
  • 期刊名称:Fresenius Environmental Bulletin
  • 印刷版ISSN:1018-4619
  • 出版年度:2019
  • 卷号:28
  • 期号:11A
  • 页码:8383-8390
  • 语种:English
  • 出版社:PSP Publishing
  • 摘要:To explore mesosulfuron-methyl (mesosulfuron) resistance and its mechanisms in Tausch's goatgrass (Aegilops tauschii Cosson) (TGA),the mesosulfuron susceptibility of 10 TGA populations from Henan Province and the effects of mesosulfuron on the target enzyme acetolactate synthase (ALS) as well as the metabolic enzyme glutathione S-transferase (GST) were evaluated. The whole plant assay results indicated that the TGA population that was least susceptible to mesosulfuron was from Huojia (Xinxiang) and that the mesosulfuron concentration required to reduce its growth by 50% (GR_(50)) was 47.26 times greater than that of the most susceptible population from Tangyin (Anyang); the GR_(50) values in these two populations were 15.12 and 0.32 g a.i. ha~(-1),respectively. In vitro ALS activity assays showed that the ALS-specific activity of the TGA population that was least susceptible to mesosulfuron from Lankao (Kaifeng) was 368.12 times greater than that of the most susceptible population from Zhongmou 2014 (Zhengzhou). The mesosulfuron concentrations required to inhibit ALS-specific activity in these populations by 50% (IC_(50)) were 900.79 and 2.45 mg L~(-1),respectively. In vitro GST activity assays indicated that the conjugate effects of mesosulfuron on GST was strongest in the goatgrass from Lankao (Kaifeng), where the effect was 148.55 times stronger than that of the most susceptible population,which was from Zhongmou 2014 (Zhengzhou); the IC_(50) values of these populations were 462.00 and 3.11 mg L~(-1), respectively. These results suggest that TGA populations from Henan Province have developed different levels of mesosulfuron resistance and that the increased specific activity and gene expression of ALS and GST may play critical roles in mesosulfuron resistance.
  • 关键词:Susceptibility;Acetolactate Synthase (ALS);Herbicide; Mesosulfuron;Tausch's Goatgrass
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