文章基本信息

标题：Carriage rate and antimicrobial resistance profiles of Staphylococcus aureus among healthcare workers at a large tertiary referral hospital in Lusaka, Zambia
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作者：Godwin Chakolwa ; Mulemba Tillika Samutela ; Geoffrey Kwenda 等
期刊名称：Scientific African
印刷版ISSN：2468-2276
出版年度：2019
卷号：5
页码：1-9
DOI：10.1016/j.sciaf.2019.e00105
语种：English
出版社：Elsevier
摘要：AbstractBackgroundStaphylococcus aureusis a frequent cause of hospital-acquired infections worldwide. The carriage ofS. aureusamong healthcare workers has been associated with transmission of the bacterium to susceptible patients.ObjectivesThe aim of this study was to determine the carriage rate, antimicrobial susceptibility profile andspatype ofS. aureusamong healthcare workers at a large tertiary referral hospital in Lusaka, Zambia.MethodsThis was a prospectivecross-sectional study that involved the collection of nasal and hand swabs from 140 healthcare workers from May to July 2017 at the University Teaching Hospital in Lusaka. In this study, we recruited 103 nurses, 28 doctors and 9 laboratory scientists. Conventional microbiological methods were used to isolate and identifyS. aureus. Antimicrobial susceptibility testing, including methicillin resistance was determined using the disc diffusion method, while molecular analysis of the isolates was achieved byspatyping.ResultsThe overallS. aureuscarriage among the health care workers was 17.1% (24/140). Of these, 13.6% (19/140) and 8.6% (12/140) were nasal and hand carriers, respectively. Carriage rate was highest among doctors (17.9%, 5/28), followed by nurses (17.5%, 18/103) and laboratory scientists (11.1%, 1/9). About 25.8% (8/31) of theS. aureusisolates were methicillin-resistant and showed resistance to more than four antibiotics. About 25.8% (8/31) of theS. aureusisolates were positive for thespagene. Of these, seven were typeable. Twospatypes, t015 (42.8%, 3/7) and t069 (14.3%, 1/7) were detected, whereas thespatype for 42.8% (3/7) of the isolates were unknown.ConclusionThe carriage ofS. aureusand prevalence of Methicillin-resistantS. aureusamong healthcare workers examined was high. This calls for regular intervention measures such as screening and decolonizing of healthcare workers to reduce the carriage and spread of this pathogen in healthcare centres in Zambia.
关键词：KeywordsAntimicrobial resistanceCarriage rateHealthcare workersMethicillin-resistantStaphylococcus aureusSpatyping