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标题：VEGF and KRAS are Potential Targets of miR-206 Modulation in Triple Negative Breast Cancer
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作者：Shaymaa E. El Feky ; Fawziya A.R. Ibrahim ; Adel Nassar 等
期刊名称：Journal of Cancer Research and Treatment
印刷版ISSN：2374-1996
电子版ISSN：2374-2003
出版年度：2019
卷号：7
期号：1
页码：10-16
DOI：10.12691/jcrt-7-1-2
语种：English
出版社：Science and Education Publishing
摘要：Triple negative is a subtype of breast cancer characterized by lack of expression of hormone receptors (ER, PR and Her2/neu). Due to the limited treatment options, the search for novel treatment targets continues. The aim of this study was to assess the differential expression of miR-206, VEGF and KRAS in TNBC and non-TNBC tissues and cell lines and to evaluate the modulatory effect of miR-206 on the key oncogenic targets VEGF and KRAS. The expression of miR-206, VEGF and KRAS was quantified using real time PCR in both paraffin embedded breast cancer and adjacent tissues as well as in MDA-MB-231 and MCF-7 cell lines. Cell lines were transfected with different concentrations of miR-206 mimic and their viability were assessed using MTT assay. Our results indicated that miR-206 was significantly downregulated in cancerous compared to non-cancerous tissues with a more pronounced downregulation in TNBC than non-TNBC tissues. VEGF and KRAS were significantly upregulated in TNBC compared to non-TNBC and their expression was negatively correlated to miR-206 expression. Transfection of TNBC and non-TNBC cell lines with miR-206 mimic resulted in a dose dependent reduction in cell viability as well as a significant reduction in VEGF and KRAS expression. In conclusion, based on our combined human tissues and cell line-based investigations we can suggest that VEGF and KRAS may be potential targets for miR-206-mediated regulation and that their targeting by miR-206 can be a highly efficient therapeutic strategy in TNBC.
关键词：triple negative breast cancer; miR-206; VEGF; KRAS; epigenetic regulation