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标题：Phytochemical Screening and Antibacterial Properties of Croton hirtus L’Her. Plant Against Some Important Pathogenic Bacteria
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作者：M. P. Subin ; Navya Reghu
期刊名称：Nature, Environment and Pollution Technology
印刷版ISSN：0972-6268
出版年度：2012
卷号：11
期号：1
语种：English
出版社：Technoscience Publications
摘要：The present study is aimed at evaluating the in vitro antimicrobial activity of different solvent extracts ofCroton hirtus L’Her. against ten medically important bacterial strains namely Escherichia coli, Enterococcussp., Salmonella typhi, Salmonella paratyphi, Bacillus thuringiensis , Bacillus subtili, Staphylococcus aureus,Pseudomonas fluorescens, Proteus sp. and Klebsiella pneumoniae. Phytochemical analysis and antimicrobialproperties of shoot, root and whole plant extracts of C. hirtus were investigated separately in methanol,ethanol, chloroform, acetone and water. Phytochemical screening reveals the presence of alkaloids, tannins,flavonoids, steroids, phenols, glycosides, terpenoids, anthroquinone, saponins and cardiac glycosides. Themethanol extract exhibited higher and wider range of activity against majority of the test organisms. Theshoot methanol extracts recorded highest activity against Bacillus thuringiensis (13.67 mm ZOI and MIC of600µg/mL), Bacillus subtili (13.83 mm ZOI and MIC of 600µg/mL) and Salmonella paratyphi (12.5 mm ZOIand MIC of 800µg/mL). The methanol whole plant extracts demonstrated highest activity against Salmonellatyphi (13.33 mm ZOI and MIC of 700µg/mL) whereas methanol root extracts demonstrated highest againstKlebsiella pneumoniae (13.33 mm ZOI and MIC of 800µg/mL) and Enterococcus sp. (10.5 mm ZOI andMIC of 1000µg/mL). The performance of methanol extracts was followed by chloroform extracts. Thechloroform whole plant extracts showed the highest activity against Escherichia coli (14.67 mm ZOI andMIC of 400µg/mL) and Staphylococcus aureus (12.67 mm ZOI and MIC of 800 µg/mL), whereas chloroformshoots extracts against Pseudomonas fluorescens (12.33 mm ZOI and MIC of 900µg/mL). The highestactivity against Proteus sp. was recorded in shoot acetone extracts (10.67 mm ZOI and MIC of 1000µg/mL).Water and ethanol extracts demonstrated the least activity against the test bacteria.