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标题：Analysis of Microbial Community in the Anaerobic Phosphorus Sludge Using Molecular Techniques
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作者：Guanhua Gao ; Hongwei Rong ; Chaosheng Zhang 等
期刊名称：Nature, Environment and Pollution Technology
印刷版ISSN：0972-6268
出版年度：2013
卷号：12
期号：1
语种：English
出版社：Technoscience Publications
摘要：Through anaerobic culture test, from the six different anaerobic phosphorus removal sludge, it was foundthat Anaerobic Sequencing Batch Reactor (ASBR) sludge is the most appropriate sludge source to removephosphorus in the liquid medium, followed by Expanded Granular Sludge Bed (EGSB) sludge and chickenmanure. Microbial community structures in the six different sludge sources were investigated by 16SPolymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) method when theanaerobic systems operated steadily. The DGGE fingerprints were analysed by the software Quantity Oneto obtain the information of the microbial species in the six different sludge sources, which showed that therewas a high diversity in the bacterial communities, and the richness value of ASBR sludge was 0.59 whosenumber occupied more than half of the total bands, while The richness value reached highest (0.61) when itwas chicken manure. The community similarity between ASBR sludge and EGSB sludge is the highest 71.7.In the bands strength schematic diagram, the No. 1, No. 8 and No. 18 band that existed in ASBR sludge,EGSB sludge and chicken manure might represent the colony related to the anaerobic phosphorus microbe.