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标题：Identification and Characterization of the Effectively Arsenic Tolerant Bacterial Strains from the Potential Arsenic Contaminated site in 24-Parganas (North) District of West Bengal, India
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作者：Debanjana Sengupta ; Arup Kumar Mitra ; Sudeshna Shyam Choudhury 等
期刊名称：Nature, Environment and Pollution Technology
印刷版ISSN：0972-6268
出版年度：2013
卷号：12
期号：2
语种：English
出版社：Technoscience Publications
摘要：Arsenic is a common menace in the state of West Bengal particularly in the district of 24-Parganas (North)and (South). The major source of arsenic being geologic in nature. Soil samples collected from one suchparticular location Baduria were tested for the presence of arsenic (As), and on an average the As contentof the soil was 2.18mg/kg. Different bacteria were isolated by serial dilution and were also tested for Astolerance. On the basis of their tolerance, three organisms (viz. C-7, A-2, A-1) have shown remarkablepercentage uptake of As 43%, 22.17% and 14% respectively, which was highest for C-7 strain. Azithromycinand Streptomycin were found to be more effective to control C-7 with an inhibition zone of 4.5 cm, but for A-2 and A-1 it was Azithromycin with inhibition zone of 3.8cm and 3.5cm respectively. These organisms werecharacterized and identified by 16S rDNA technique, BLAST results and the cluster analysis by NTSYSpcver. 2.02e program, and found to be Bacillus sp. strain CCBAU 51490 (C-7) (GenBank Accession NumberEF377314.1), Bacillus subtilis sub sp. subtilis strain DSM 10 (Genbank Accession Number NR_027552.1)(A-2) and Pseudomonas otidis strain MCC 10330 (Genbank Accession Number NR_043289.1) (A-1). Theycan be used in the treatment of As contaminated sludge generated industrially from As treatment plant. Thiscan give a new insight in microbial arsenic trapping with minimum toxic implications in the nature.