文章基本信息

标题：Characterization of a Bioactive Compounds Produced by Streptomyces phaeochromogenes NRRL B-2123
本地全文：下载
作者：Zahra Bamzadeh ; Majid Baserisalehi ; Nima Bahador 等
期刊名称：Nature, Environment and Pollution Technology
印刷版ISSN：0972-6268
出版年度：2014
卷号：13
期号：1
语种：English
出版社：Technoscience Publications
摘要：Streptomyces is the largest genus of Actinobacteria and includes aerobic, Gram-positive filamentous bacteriawith high G+C content. Although, few species of Streptomyces are pathogens, they could be characterizedby production of the beneficial metabolites viz., antibiotics, antifungal, antiparasitic, and immunosuppressantcompounds. The present study was conducted to isolate Streptomyces from soil and characterize theirbioactive compounds. In total 85 soil samples were collected and assessed for production of the bioactivecompounds by Agar Well Diffusion method against antagonistic microorganisms such as Escherichia coli,Pseudomonas aeruginosa, Bacillus cereus, Staphylococcus aureus, Aspergillus niger and Candida albicans.Of all the Streptomyces isolates, one strain exhibited potent activity against Staphylococcus aureus, Bacilluscereus and Aspergillus niger. The promising strain identified using 16SrRNA gene sequencing and recognizedas Streptomyces phaeochromogenes strain NRRL. Then, the growth phase of production and ArbitraryUnit (AU) of the bioactive compounds were determined, and the cured bioactive compound was purified andsubjected to 1H NMR, 13C NMR and FTIR analysis for determination of the structural formula. The resultsobtained illustrated that high level of the bioactive compounds produced at stationary phase with ArbitraryUnit 32 AU. On the other hand, the data obtained from 1H NMR, 13C NMR and FTIR analysis exhibited astraight chain with a possible structural formula C 13 H 15 NO 3 for the bioactive compound.