文章基本信息

标题：Modification in Asphalt Texture by Heavy Metal Tolerant Bacteria Isolated From Industrial Effluent
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作者：Moumita Dasgupta ; Rashmi Das ; Ghazala Haque 等
期刊名称：Nature, Environment and Pollution Technology
印刷版ISSN：0972-6268
出版年度：2014
卷号：13
期号：4
语种：English
出版社：Technoscience Publications
摘要：Asphalt is a sticky, black and highly viscous liquid or semi-solid form of petroleum. It is also known asbitumen which may be found in natural deposits or may be a refined product; classed as a pitch. Earlierreports show that most natural bitumen contains sulphur and several heavy metals, such as nickel,vanadium, lead, chromium, mercury, arsenic, selenium, and other toxic elements. The primary use ofasphalt/bitumen is in road construction, where it is used as the glue or binder mixed with aggregateparticles to create asphalt concrete. Many studies demonstrated microbial utilization of asphalt andrelated hydrocarbons, particularly the petroleum hydrocarbons. Investigation was carried out to isolateand characterize microorganisms and determine their heavy metal tolerance activity from such kind ofponds in which the pitch waste effluent was released. Later, it was checked whether they have any rolein the modification of asphalt texture. Experiments were executed by first determining the physical andchemical characteristics (flame test) of the pitch effluent, then isolating the microorganisms from it andfound Gram positive Coccus. Flame test revealed the presence of arsenic (As) and lead (Pb). Then, itwas checked whether the isolated microorganisms are tolerant to these heavy metals. Later, the use ofthese heavy metals by the isolated microorganisms was detected by Atomic AbsorptionSpectrophotometric study. The pattern of growth indicated it to be Staphylococcus sp., and whenincubated with asphalt, it not only utilizes the hydrocarbon, but also prevents it from hardening. Thus, apathogenic bacteria can be utilized in recycling of complex hydrocarbon and it continue to sustain itselffor a prolong period even in the absence of suitable substrate.