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  • 标题:Diversity, Identification and Biotyping of Bacillus thuringiensis Strains from Soil Samples in Iran
  • 本地全文:下载
  • 作者:Elham Moazamian ; Nima Bahador ; Manoochehr Rasouli
  • 期刊名称:Nature, Environment and Pollution Technology
  • 印刷版ISSN:0972-6268
  • 出版年度:2016
  • 卷号:15
  • 期号:3
  • 语种:English
  • 出版社:Technoscience Publications
  • 摘要:Bacillus thuringiensis is a bacterium known for producing insecticidal and cytocidal crystal protein duringsporulation. One hundred soil samples were analysed which were introduced by seven methods, out ofwhich sixty B. thuringiensis strains were collected to study the distribution and diversity of crystal proteins.The strains were characterized on the basis of microscopic examination of parasporal crystal morphology,biochemical tests, and analysis of parasporal inclusions protein profiles by SDS-PAGE. Majority of strainshad bipyramidal crystals. B. thuringiensis subsp. thuringiensis was the most common biochemical type.Overall, according to our finding, it could be concluded that genetic diversity of B. thuringiensis strains isdependant on the geographical areas. It means that the production of crystal protein by different strains of B.thuringiensis probably dependant on the geographical area. .
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