标题:Effect of Immobilized Bacteria on TAN Suppression, Survival Rate, and Biomass Harvest of Pacific White Shrimp, Litopenaeus vannamei, Grown in Biofloc Culture System
摘要:In this study, immobilized bacteria (Nitrosomonas, Nitrobacter and Nitrospira) were added into threeshrimp ponds in a concentration of 2106 cfu mL-1 that equals to 20 kg beads ha-1. The other threeponds were used as control treatment. All ponds were stocked with PL’s (Post Larvae) of Pacificwhite shrimp at a density of 100 PL’s m-2. Shrimps were fed with a commercial diet containing 36%crude protein, 7% fat, 10% ash, and 2% crude fibre. Oxygen was kept near saturation throughpaddle-wheels. Secchi disc depth, pH and temperature were monitored daily. Total ammonia nitrogen(TAN), nitrite and nitrate were measured every 10 days together with sampling for shrimp body weightand feed adaptation. Average TAN concentration of ponds with nitrifying bacteria was 0.12±0.144 mgL-1, one fourth of the control ponds (0.523±0.564 mg L-1). Average nitrite concentration in control ponds(0.058±0.045 mg L-1) was also significantly higher than the ponds added with immobilized bacteria(0.037±0.038 mg L-1). However, the end product of nitrate was not significantly different ( = 0.607)among the ponds. Although not affecting shrimp growth, TAN suppression significantly increases thesurvival rate and finally resulted in higher ( = 0.043) biomass harvest, from 12.37 t ha-1 (controlponds) to 18.87±2.91 t ha-1 at treatment ponds. This study concluded the importance of transformingTAN concentration into nitrate, particularly in intensive culture system. To do this, the presence ofimmobile nitrifying bacteria is needed.