文章基本信息

标题：Outer-membrane translocation of bulky small molecules by passive diffusion
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作者：Bert van den Berg ; Satya Prathyusha Bhamidimarri ; Jigneshkumar Dahyabhai Prajapati 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2015
卷号：112
期号：23
页码：E2991-E2999
DOI：10.1073/pnas.1424835112
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：SignificanceThe outer membrane (OM) of gram-negative bacteria forms a protective layer on the outside of the cell that prevents unrestricted access of harmful compounds. For the acquisition of ions and nutrients, the OM contains two types of transport proteins: passive diffusion channels and active transporters. Due to the limited diameters of passive diffusion channels, bulky molecules such as iron-siderophores and complex oligosaccharides are assumed to be taken up exclusively by active transporters. Here we assert that this assumption is incorrect. Using a combination of biophysical and computational approaches, we show that the OM protein CymA (cyclodextrin metabolism A) from Klebsiella oxytoca represents a previously unidentified paradigm in OM transport by mediating the passive diffusion of cyclic oligosaccharides (cyclodextrins) with diameters of [~]15 [IMG]f1.gif" ALT="A" BORDER="0">. The outer membrane (OM) of gram-negative bacteria forms a protective layer around the cell that serves as a permeability barrier to prevent unrestricted access of noxious substances. The permeability barrier of the OM results partly from the limited pore diameters of OM diffusion channels. As a consequence, there is an "OM size-exclusion limit," and the uptake of bulky molecules with molecular masses of more than [~]600 Da is thought to be mediated by TonB-dependent, active transporters. Intriguingly, the OM protein CymA from Klebsiella oxytoca does not depend on TonB but nevertheless mediates efficient OM passage of cyclodextrins with diameters of up to [~]15 [IMG]f1.gif" ALT="A" BORDER="0">. Here we show, by using X-ray crystallography, molecular dynamics simulations, and single-channel electrophysiology, that CymA forms a monomeric 14-stranded {beta}-barrel with a large pore that is occluded on the periplasmic side by the N-terminal 15 residues of the protein. Representing a previously unidentified paradigm in OM transport, CymA mediates the passive diffusion of bulky molecules via an elegant transport mechanism in which a mobile element formed by the N terminus acts as a ligand-expelled gate to preserve the permeability barrier of the OM.
关键词：CymA ; outer membrane channel ; cyclodextrin ; passive diffusion ; ligand gating