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标题：The effect of high dietary fructose on the kidney of adult albino rats and the role of curcumin supplementation: A biochemical and histological study
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作者：Samraa H. Abdel-Kawi ; Kamel M.A. Hassanin ; Khalid S. Hashem 等
期刊名称：Beni-Suef University Journal of Basic and Applied Sciences
印刷版ISSN：2314-8535
电子版ISSN：2314-8543
出版年度：2016
卷号：5
期号：1
页码：52-60
DOI：10.1016/j.bjbas.2015.12.002
语种：English
出版社：Elsevier
摘要：BackgroundConsumption of fructose, in the form of added sugars such as high fructose corn syrup (HFCS) or sucrose, has increased markedly in the last few years, which is strongly correlated with the prevalence of metabolic syndrome. It is widely used as a food ingredient and has potential to increase oxidative stress. Curcumin is a phenolic compound, and it exhibits protective effects against oxidative damage.ObjectiveThe aim of the study was to investigate the effects of curcumin on renal injury in fructose-fed rats and the possible underlying mechanism.MethodsEighty male rats were randomly divided into control group, fructose group, 200 mg/kg curcumin group and curcumin-fructose group. The histopathological changes in the kidney of rats were observed using hematoxylin and eosin and Masson's trichrome stains. The expressions of renal reduced glutathione (GSH) concentration, glutathione reductase (GR), super oxide dismutase (SOD), catalase activities, lipid peroxidation (LPO), DNA fragmentation %, inducible nitrous oxide (INOS) and homooxygenase 1 (HO-1) mRNA in renal tissue homogenates were assessed. Immunohistochemical detection of alpha-smooth muscle actin (α-SMA) and tumor necrosis factor alpha (TMF-α) were also investigated.ResultsCompared to the control group, GSH, GR, SOD and catalase activities were significantly decreased in the fructose group, while there was a significant increase in LPO, DNA fragmentation %, INOS and HO-1. These changes were accompanied by renal tubular injury, increased collagen deposition and lipid accumulation. Immunohistochemical results revealed increased expression of both α-SMA and TNF-α. Curcumin at 200 mg/kg evidently improved renal tubular injury, suppressed the expressions of renal LPO, DNA fragmentation %, INOS and HO-1, and decreased the expression of both α-SMA and TNF-α in kidney tissue.ConclusionCurcumin administration protected the kidney cells from fructose induced oxidative stress by increasing the antioxidant defence mechanism of the kidney cells and its ability to act as a free radical scavenger.
关键词：Fructose;Renal oxidative stress;Curcumin;HO- 1;INOS;TNF-α and α-SMA