摘要:In the present report, we investigated changes in oxidative stress parameters, photosynthetic properties and the chloroplast ultrastructure of Taxiphyllum taxirameum during short-term Cu and Cr exposure. The results showed that treatment of Cu and Cr for 72h (100 µM concentration) significantly increased the formation of reactive oxygen species (ROS) and lipid peroxidation, while total chlorophyll (Chl) content decreased and a decline in peroxidase (POD) activities. However, superoxide dismutase (SOD) and catalase (CAT) activities exposed different patters after short-term exposure to different concentrations of Cu and Cr. Changes in chlorophyll fluorescence (Fv/Fm, qP and NPQ) indicated that heavy metal treatment decreased photosystem II functions. The ultrastructural studies showed no significant ultrastructural changes at the high concentrations (100 µM) of Cu and Cr in treatment solutions. These experiments suggest that oxidative stress parameters and chlorophyll fluorescence could be used as an effective indicator in moss biomonitoring and T. taxirameum might have a potential tolerance to heavy metals.
关键词:Antioxidants; Bryophytes; Cu and Cr stress; Chlorophyll fluorescence; Ultrastructure