摘要:In order to evaluate the distribution characteristics of microbial populations involved in nitrogen removal, field experiments of a subsurface wastewater infiltration system (8 sub-cells, length × width = 20 m × 15 m for each subcell) were carried out for 360 days. The results indicated that hydraulic and pollutant loading rate, oxygen reduction potential and wastewater shunt distribution have a significant effect on the distributions of nitrifying and denitrifying bacteria. For nitrifying bacteria, hydraulic and pollutant loading rates, oxygen reduction potential and carbon source supplement method have negative influence on its population, especially near the wastewater distribution area. The changes of denitrifying bacteria distribution are opposite to those of nitrifying bacteria. Taking into account the nitrogen removal efficiency, hydraulic and pollutant loading rate are proposed within a range from 0.065 to 0.081 m3/ m2·d and 9.3 to 16.8 g BOD/m2·d, respectively. Intermittent operation mode is recommended and carbon source is suggested to introduce into the 65 cm depth.