文章基本信息

标题：Se-SAD serial femtosecond crystallography datasets from selenobiotinyl-streptavidin
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作者：Chun Hong Yoon ; Hasan DeMirci ; Raymond G. Sierra 等
期刊名称：Scientific Data
电子版ISSN：2052-4463
出版年度：2017
卷号：4
DOI：10.1038/sdata.2017.55
语种：English
出版社：Nature Publishing Group
摘要：We provide a detailed description of selenobiotinyl-streptavidin (Se-B SA) co-crystal datasets recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS) for selenium single-wavelength anomalous diffraction (Se-SAD) structure determination. Se-B SA was chosen as the model system for its high affinity between biotin and streptavidin where the sulfur atom in the biotin molecule (C10H16N2O3S) is substituted with selenium. The dataset was collected at three different transmissions (100, 50, and 10%) using a serial sample chamber setup which allows for two sample chambers, a front chamber and a back chamber, to operate simultaneously. Diffraction patterns from Se-B SA were recorded to a resolution of 1.9鈥壝? The dataset is publicly available through the Coherent X-ray Imaging Data Bank (CXIDB) and also on LCLS compute nodes as a resource for research and algorithm development.